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Product Info Summary
| SKU: | EKC1737 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
MAT1 Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1737
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The MAT1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MAT1 protein expression profile in cells. The kit can be used for measuring the relative amounts of MAT1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MAT1.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
MAT1 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1737)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1737 is reactive to MNAT1 in Human, Mouse, Rat samples
Application Guarantee
EKC1737 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-MNAT1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa/HepG2/293 cells
Protein Target Info & Infographic
Gene/Protein Information For MNAT1 (Source: Uniprot.Org, NCBI)
Gene Name
MNAT1
Full Name
CDK-activating kinase assembly factor MAT1
Weight
35.823kDa
Alternative Names
CAP35; CDK7/cyclin-H assembly factor; cyclin G1 interacting protein; Cyclin-G1-interacting protein; MAT1TFB3; menage a trois 1 (CAK assembly factor); menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis); Menage a trois; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66CDK-activating kinase assembly factor MAT1 MNAT1 CAP35, MAT1, RNF66, TFB3 MNAT1 component of CDK activating kinase CDK-activating kinase assembly factor MAT1|CDK7/cyclin-H assembly factor|MNAT CDK-activating kinase assembly factor 1|MNAT1, CDK activating kinase assembly factor|RING finger protein 66|RING finger protein MAT1|cyclin G1 interacting protein|menage a trois 1 (CAK assembly factor)|menage a trois homolog 1, cyclin H assembly factor|menage a trois-like protein 1 cyclin H assembly factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MNAT1, check out the MNAT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MNAT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MAT1 Colorimetric Cell-Based ELISA (EKC1737)
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